	US 12,084,704 B2
	Methods for producing isobutene from 3-methylcrotonic acid
Romain Chayot, Paris (FR); Mathieu Allard, Saint-Vrain (FR); and Maria Anissimova, Nozay (FR)
Assigned to Global Bioenergies, Evry (FR)
Appl. No. 16/612,065
Filed by Global Bioenergies, Evry (FR)
PCT Filed Apr. 19, 2018, PCT No. PCT/EP2018/060051 § 371(c)(1), (2) Date Nov. 8, 2019, PCT Pub. No. WO2018/206262, PCT Pub. Date Nov. 15, 2018.
Claims priority of application No. 17170429 (EP), filed on May 10, 2017.
Prior Publication US 2021/0277425 A1, Sep. 9, 2021
Int. Cl. C12P 5/02 (2006.01)

CPC C12P 5/026 (2013.01) [C12Y 205/01 (2013.01); C12Y 207/0105 (2013.01); C12Y 207/04026 (2015.07); C12Y 207/06002 (2013.01); C12Y 207/06003 (2013.01); C12Y 401/01 (2013.01); C12Y 503/03002 (2013.01)]

20 Claims

1. A method for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with a prokaryotic FMN prenyl transferase,

wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically by:

(i) the enzymatic conversion of isopentenyl pyrophosphate (IPP) into said DMAPP; or

(ii) the enzymatic conversion of dimethylallyl phosphate (DMAP) into said DMAPP; or

(iii) the enzymatic conversion of prenol into said DMAPP;

(iv) or by a combination of any one of (i) to (iii).