US 12,084,672 B2
Genetically engineered land plants that express an increased seed yield protein and/or an increased seed yield RNA
Jihong Tang, West Roxbury, MA (US); Meghna Malik, Saskatoon (CA); Nirmala Sharma, Saskatoon (CA); Claire Burkitt, Saskatoon (CA); Yuanyuan Ji, Saskatoon (CA); Madana M. R. Ambavaram, Andover, MA (US); Kieran Ryan, Sharon, MA (US); Oliver P. Peoples, Arlington, MA (US); Kristi D. Snell, Belmont, MA (US); Frank Anthony Skraly, Watertown, MA (US); and Venkatesh Bollina, Saskatoon (CA)
Assigned to Yield10 Bioscience, Inc., Woburn, MA (US)
Appl. No. 17/273,159
Filed by YIELD10 BIOSCIENCE, INC., Woburn, MA (US)
PCT Filed Sep. 3, 2019, PCT No. PCT/US2019/049281
§ 371(c)(1), (2) Date Mar. 3, 2021,
PCT Pub. No. WO2020/051108, PCT Pub. Date Mar. 12, 2020.
Claims priority of provisional application 62/834,766, filed on Apr. 16, 2019.
Claims priority of provisional application 62/744,932, filed on Oct. 12, 2018.
Claims priority of provisional application 62/726,653, filed on Sep. 4, 2018.
Prior Publication US 2021/0348182 A1, Nov. 11, 2021
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01)
CPC C12N 15/8262 (2013.01) [C07K 14/415 (2013.01); C12N 15/8249 (2013.01)] 8 Claims
 
1. A genetically engineered land plant that expresses a protein that increases seed yield with increased expression (“an ISY protein”), the genetically engineered land plant comprising a modified gene for the ISY protein, wherein:
the ISY protein comprises SEQ ID NO: 2;
the ISY protein has homology to a plant invertase inhibitor and/or a pectin methylesterase inhibitor;
the modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the ISY protein;
the promoter is non-cognate with respect to the nucleic acid sequence encoding the ISY protein;
the modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the ISY protein; and
the genetically engineered land plant is Camelina sativa, canola, or soybean.