	US 12,084,671 B2
	Optimized CRISPR-Cas nucleases and base editors and methods of use thereof
Nathaniel Graham, Durham, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Filed by Pairwise Plants Services, Inc., Durham, NC (US)
Filed on Feb. 6, 2023, as Appl. No. 18/164,910.
Application 18/164,910 is a continuation of application No. 17/078,576, filed on Oct. 23, 2020, granted, now 11,591,607.
Claims priority of provisional application 62/925,422, filed on Oct. 24, 2019.
Prior Publication US 2023/0183726 A1, Jun. 15, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/8218 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 2310/20 (2017.05)]

19 Claims

1. A nucleic acid construct encoding a CRISPR-Cas nuclease operably associated with a promoter region, wherein the promoter region comprises an intron, optionally wherein the promoter region comprises a ubiquitin promoter and intron, wherein the nucleic acid construct encoding the CRISPR-Cas nuclease comprises the nucleotide sequence of SEQ ID NO:5.