US 12,084,666 B2
Compositions and methods of dual poison-antidote meiotic drivers
Sarah E. Zanders, Prairie Village, KS (US); Nicole Nuckolls, Shawnee, KS (US); Maria Angelical Bravo Nunez, Kansas City, KS (US); and Harmit Singh Malik, Shoreline, WA (US)
Assigned to STOWERS INSTITUTE FOR MEDICAL RESEARCH, Kansas City, MO (US); and FRED HUTCHINSON CANCER CENTER, Seattle, WA (US)
Appl. No. 16/608,146
Filed by STOWERS INSTITUTE FOR MEDICAL RESEARCH, Kansas City, KS (US); and FRED HUTCHINSON CANCER CENTER, Seattle, WA (US)
PCT Filed Apr. 27, 2018, PCT No. PCT/US2018/029997
§ 371(c)(1), (2) Date Oct. 24, 2019,
PCT Pub. No. WO2018/201073, PCT Pub. Date Nov. 1, 2018.
Claims priority of provisional application 62/491,107, filed on Apr. 27, 2017.
Prior Publication US 2020/0149054 A1, May 14, 2020
Int. Cl. C12N 15/81 (2006.01); C07K 14/39 (2006.01); C12N 1/00 (2006.01)
CPC C12N 15/815 (2013.01) [C07K 14/39 (2013.01); C12N 1/00 (2013.01); C12N 2800/00 (2013.01); C12N 2840/007 (2013.01)] 6 Claims
 
1. A meiotic drive composition comprising:
a recombinant DNA sequence encoding a first peptide sequence and a second peptide sequence, the first peptide sequence capable of destroying a gamete and the second peptide sequence capable of rescuing a gamete from the first peptide sequence;
wherein the first peptide sequence is SEQ ID NO: 3;
wherein the second peptide sequence is SEQ ID NO: 4;
wherein the recombinant DNA sequence comprises a heterologous regulatory sequence;
wherein the first peptide sequence is transported outside of a cell and the second peptide sequence is not transported outside of a cell
wherein the first peptide sequence and the second peptide sequence are derived from alternative transcriptional start sites on the recombinant DNA sequence; and
wherein the recombinant DNA sequence, when expressed in a diploid organism, is effective to bias offspring toward having the recombinant DNA.