US 12,084,524 B1
Method for extracting chondroitin sulfate from eggshell membrane
Meiyu Liu, Handan (CN); Hengyang Liu, Handan (CN); Haiping Lian, Handan (CN); Yuxin Yin, Handan (CN); Shaoqi Song, Handan (CN); and Xiaoxu Guo, Handan (CN)
Assigned to HEBEI UNIVERSITY OF ENGINEERING, Handan (CN)
Filed by Hebei University of Engineering, Handan (CN)
Filed on Apr. 28, 2024, as Appl. No. 18/648,414.
Application 18/648,414 is a continuation of application No. PCT/CN2023/141921, filed on Dec. 26, 2023.
Claims priority of application No. 202310041266.X (CN), filed on Jan. 13, 2023.
Int. Cl. C08B 37/00 (2006.01)
CPC C08B 37/0069 (2013.01) 4 Claims
 
1. A method for extracting chondroitin sulfate from eggshell membrane, comprising following steps: adding a mixed reagent into ultramicro-pulverized eggshell membrane, followed by water bath extraction; and centrifuging an extractive solution to obtain the chondroitin sulfate;
the mixed reagent is a mixed solution of alkali reagent and salt reagent, wherein the alkali reagent is a sodium hydroxide solution with a volume fraction of 2%, and the salt reagent is a 2% sodium chloride solution by volume;
an alkali-salt ratio of the alkali reagent to the salt reagent is 6 mL:1 mL;
a material-liquid ratio of the mixed reagent to the ultramicro-pulverized eggshell membrane is 1 g:15.5 mL;
a temperature of the water bath extraction is 59° C. and a duration is 7.3 h.