US 12,084,480 B2
Unstructured non-repetitive polypeptides having lcst behavior
Ashutosh Chilkoti, Durham, NC (US); Nicholas Tang, Durham, NC (US); and Garrett Kelly, Durham, NC (US)
Assigned to Duke University, Durham, NC (US)
Filed by Duke University, Durham, NC (US)
Filed on Sep. 16, 2021, as Appl. No. 17/477,192.
Application 17/477,192 is a continuation of application No. 16/335,734, granted, now 11,155,584, previously published as PCT/US2017/052887, filed on Sep. 22, 2017.
Claims priority of provisional application 62/399,123, filed on Sep. 23, 2016.
Prior Publication US 2022/0098248 A1, Mar. 31, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/31 (2006.01); A61K 38/00 (2006.01); A61K 47/65 (2017.01); G01N 33/68 (2006.01)
CPC C07K 14/31 (2013.01) [A61K 47/65 (2017.08); G01N 33/68 (2013.01); A61K 38/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/21 (2013.01); G01N 2800/52 (2013.01)] 14 Claims
 
1. An unstructured polypeptide having a lower critical solution temperature (LCST), an upper critical solution temperature (UCST), or a combination thereof,
wherein the unstructured polypeptide lacks a serial pentapeptide repeat of an elastin-like polypeptide (ELP), wherein the ELP repeat has the sequence (VPGXG)n, where n is an integer greater than 1 and X is any amino acid except proline,
wherein the unstructured polypeptide has a linguistic complexity score, and wherein the product of the linguistic complexity score and the amino acid sequence length of the unstructured polypeptide is greater than 15;
wherein the polypeptide comprises a sequence of at least 50 amino acids, wherein at least 10% of the amino acids are proline (P), and wherein at least 20% of the amino acids are glycine (G),
wherein the polypeptide phase transitions above the LCST, phase transitions below the UCST, or a combination thereof, and
wherein the LOST and the UCST are each independently from about 0° C. to about 100° C.