US 12,084,477 B2
Protein pores
Han Remaut, Ghent (BE); Sander Egbert Van Der Verren, Serskamp (BE); Nani Van Gerven, Huizingen (BE); Lakmal Nishantha Jayasinghe, Oxford (GB); Elizabeth Jayne Wallace, Oxford (GB); Pratik Raj Singh, Oxford (GB); Richard George Hambley, Oxford (GB); Michael Robert Jordan, Oxford (GB); and John Joseph Kilgour, Oxford (GB)
Assigned to VIB VZW, Ghent (BE); Vrije Universiteit Brussel, Brussels (BE); and Oxford Nanopore Technologies PLC, Oxford (GB)
Filed by VIB VZW, Ghent (BE); Vrije Universiteit Brussel, Brussels (BE); and Oxford Nanopore Technologies PLC, Oxford (GB)
Filed on Sep. 16, 2022, as Appl. No. 17/933,061.
Application 17/933,061 is a continuation of application No. 16/624,341, granted, now 11,572,387, previously published as PCT/GB2018/051858, filed on Jul. 2, 2018.
Claims priority of application No. 17179099 (EP), filed on Jun. 30, 2017.
Prior Publication US 2023/0079731 A1, Mar. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/00 (2006.01); B82Y 15/00 (2011.01); C07K 14/00 (2006.01)
CPC C07K 14/00 (2013.01) [B82Y 15/00 (2013.01)] 28 Claims
 
1. A pore comprising a CsgG pore and a CsgF peptide, wherein the CsgF peptide is bound to CsgG and forms a constriction in the pore, wherein the CsgF peptide is a truncated CsgF peptide lacking the C-terminal head domain of CsgF and at least part of the neck domain of CsgF, wherein the CsgF peptide is a homologue of SEQ ID NO: 6, and wherein the CsgF peptide comprises one or more amino acid substitutions relative to SEQ ID NO: 6.