US 12,083,512 B2
Microfluidic analysis of ligand induced cell expression
Hongxing Hu, Heidelberg (DE); Samantha Seah, Heidelberg (DE); and Christoph A. Merten, Heidelberg (DE)
Assigned to EUROPEAN MOLECULAR BIOLOGY LABORATORY, (DE)
Appl. No. 16/069,824
Filed by European Molecular Biology Laboratory, Heidelberg (DE)
PCT Filed Jan. 13, 2017, PCT No. PCT/EP2017/050620
§ 371(c)(1), (2) Date Jul. 12, 2018,
PCT Pub. No. WO2017/121832, PCT Pub. Date Jul. 20, 2017.
Claims priority of application No. 16151298 (EP), filed on Jan. 14, 2016.
Prior Publication US 2019/0022645 A1, Jan. 24, 2019
Int. Cl. B01L 3/00 (2006.01); C07K 16/28 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01); C40B 20/08 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502761 (2013.01); C07K 16/28 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6846 (2013.01); C12Q 1/686 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/021 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01); C40B 20/08 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A plurality of microfluidic compartments, wherein at least 1% of said compartments form a subset in which each compartment is a droplet comprising:
(i) a first cell,
(ii) one second cell, the second cell expressing a polypeptide ligand intended to specifically bind to a molecule or part thereof accessible on a surface of the first cell, wherein the one second cell is a cell of B-cell lineage and
(iii) a set of barcode oligonucleotides each comprising a barcode sequence unique to the set and a sequence capable of binding specifically to mRNA and/or cDNA of the first cell and mRNA and/or cDNA of the one second cell.