	US 12,083,244 B2
	Cross-linked dehydrated placental tissue grafts and methods for making and using the same
Tom Koob, Marietta, GA (US); John Daniel, Marietta, GA (US); and Randall Spencer, Marietta, GA (US)
Assigned to MiMedx Group, Inc., Marietta, GA (US)
Filed by MiMedx Group, Inc., Marietta, GA (US)
Filed on Sep. 15, 2016, as Appl. No. 15/267,026.
Application 15/267,026 is a division of application No. 13/719,150, filed on Dec. 18, 2012, granted, now 9,463,206.
Claims priority of provisional application 61/579,206, filed on Dec. 22, 2011.
Prior Publication US 2017/0000927 A1, Jan. 5, 2017
Int. Cl. A61L 27/36 (2006.01); A61K 35/50 (2015.01); A61L 15/40 (2006.01); A61L 27/50 (2006.01); A61L 27/54 (2006.01); A61L 27/60 (2006.01)

CPC A61L 27/3604 (2013.01) [A61K 35/50 (2013.01); A61L 15/40 (2013.01); A61L 27/3687 (2013.01); A61L 27/50 (2013.01); A61L 27/54 (2013.01); A61L 27/60 (2013.01); A61L 2430/40 (2013.01)]

23 Claims

1. A method for producing a dehydrated, cross-linked placental tissue graft, the method consisting of

i) contacting a dehydrated placental tissue graft with a solution comprising a cross-linking agent, thereby forming a cross-linked placental tissue graft; and

ii) dehydrating the cross-linked placental tissue graft formed in step 1), thereby forming the dehydrated, cross-linked placental tissue graft;

wherein the dehydrated placental tissue graft comprises at least two layers of amnion, or at least one layer of each of chorion and amnion; and

wherein at least one amnion layer comprises an amniotic fibroblast cell layer.