US 12,083,166 B2
Pharmaceutical composition including laminin fragments to treat or prevent the disease, disorder or symptom of tooth dentin and/or dental pulp
Jia Tang, Sapporo (JP); and Takashi Saito, Ebetsu (JP)
Filed by Takashi Saito, Ebetsu (JP)
Filed on Nov. 18, 2022, as Appl. No. 18/057,110.
Application 18/057,110 is a division of application No. 16/825,116, filed on Mar. 20, 2020, abandoned.
Claims priority of provisional application 62/821,023, filed on Mar. 20, 2019.
Prior Publication US 2023/0190882 A1, Jun. 22, 2023
Int. Cl. A61K 38/39 (2006.01); A61K 33/42 (2006.01); A61K 38/01 (2006.01)
CPC A61K 38/39 (2013.01) [A61K 33/42 (2013.01); A61K 38/014 (2013.01)] 1 Claim
 
1. A tooth dentin, pulp or pulp tissue composition for treating or preventing a disease, disorder, or symptom of a tooth dentin and/or pulp or pulp tissue, the composition comprising:
a mixture of odontoblasts and mineralized nodules secreted from the odontoblasts,
wherein the odontoblasts have been cultured on a coating which comprises an integrin binding fragment of Laminin such that mRNA expression levels of ITGA1, ITGA5, ITGAV, ITGB1, and ITGB5 in the odontoblasts are increased as compared with the odontoblasts which have not been cultured on the coating,
wherein the Laminin is at least one of Laminin 511 and Laminin 411, the integrin binding fragment comprising an integrin-binding domain, and a coating density of the integrin binding fragment of the at least one of Laminin 511 and the Laminin 411 in the coating is 1 to 8 μg/cm2, and
wherein the composition is applicable to a tooth and able to treat or prevent the disease, disorder or symptom of the tooth dentin and/or pulp tissue including dental caries when applied to the tooth.