US 12,083,159 B2
Antibacterial compositions, methods of making and use thereof
Vincent Craig Bond, Stone Mountain, GA (US); Michael Powell, Douglasville, GA (US); Ming Bo Huang, Atlanta, GA (US); Syed Ali, New Iberia, LA (US); and Martin N. Shelton, Seattle, WA (US)
Assigned to MOREHOUSE SCHOOL OF MEDICINE, Atlanta, GA (US)
Filed by MOREHOUSE SCHOOL OF MEDICINE, Atlanta, GA (US)
Filed on Mar. 6, 2020, as Appl. No. 16/811,754.
Application 16/811,754 is a continuation of application No. 16/226,242, filed on Dec. 19, 2018.
Application 16/226,242 is a continuation in part of application No. 15/397,359, filed on Jan. 3, 2017, granted, now 10,206,974, issued on Feb. 19, 2019.
Application 15/397,359 is a continuation of application No. 14/156,119, filed on Jan. 15, 2014, granted, now 9,556,224, issued on Jan. 31, 2017.
Application 14/156,119 is a continuation of application No. 13/267,977, filed on Oct. 7, 2011, granted, now 8,669,226, issued on Mar. 11, 2014.
Claims priority of provisional application 62/678,002, filed on May 30, 2018.
Prior Publication US 2020/0197483 A1, Jun. 25, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/16 (2006.01); A61K 38/08 (2019.01); A61K 45/06 (2006.01); A61K 47/60 (2017.01); A61K 47/64 (2017.01); C07K 7/06 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01)
CPC A61K 38/162 (2013.01) [A61K 38/08 (2013.01); A61K 45/06 (2013.01); A61K 47/60 (2017.08); A61K 47/6455 (2017.08); C07K 7/06 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C07K 2319/10 (2013.01); C12N 2740/16032 (2013.01); C12N 2740/16322 (2013.01); Y02A 50/30 (2018.01)] 5 Claims
 
1. A method of detaching a gram-positive or gram-negative bacterial biofilm from a surface or from other microorganisms, comprising
contacting said surface or said other microorganisms with a composition comprising an antimicrobial SMR peptide comprising an HIV-1 SMRwt peptide and a cell penetrating peptide (CPP) domain, wherein the antimicrobial SMR peptide consists of the amino acid sequence of SEQ ID NO: 9;
wherein the composition is incorporated in a biologically compatible anti-biofilm material selected from the group consisting of paint, glue, and cement;
wherein the anti-biofilm paint, glue, or cement comprises the SMR peptide SEQ ID NO: 9 in an effective concentration to inhibit biofilm formation and detach a biofilm from a surface or from other microorganisms;
and wherein the composition does not contain a bacterial biofilm-degrading enzyme.