US 12,083,095 B2
Method for purifying cannabinoids
Jin Seok Hur, Glen Mills, PA (US)
Assigned to SARTORIUS CHROMATOGRAPHY EQUIPMENT, Pompey (FR)
Filed by Sartorius Chromatography Equipment, Pompey (FR)
Filed on Apr. 4, 2022, as Appl. No. 17/657,826.
Application 17/657,826 is a continuation of application No. 16/155,646, filed on Oct. 9, 2018, granted, now 11,324,718.
Prior Publication US 2022/0218656 A1, Jul. 14, 2022
Int. Cl. A61K 31/352 (2006.01); A61K 36/185 (2006.01); B01D 15/18 (2006.01); B01D 15/32 (2006.01); C07K 1/22 (2006.01); G01N 30/02 (2006.01); G01N 30/06 (2006.01)
CPC A61K 31/352 (2013.01) [A61K 36/185 (2013.01); B01D 15/1871 (2013.01); B01D 15/322 (2013.01); C07K 1/22 (2013.01); G01N 30/02 (2013.01); B01D 15/1821 (2013.01); G01N 2030/065 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of:
injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles in a chromatographic installation having a non-static bed;
performing an elution with an elution solution, and collecting one or more elution fractions; and
optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions; wherein
at least one of the elution fractions or washing fractions contains the at least one cannabinoid compound purified from the one or more additional compounds,
the elution solution is a mixture of a polar solvent and a non-polar solvent, the polar solvent being an alcohol and the non-polar solvent being an alkane, and
the elution is performed in an isocratic mode, and
the method further comprises a preliminary purification stage before the main purification stage, said preliminary purification stage comprising the steps of:
putting a preliminary mixture comprising the at least one cannabinoid compound, the additional compounds and one or more further compounds in contact with a preliminary stationary phase; and
collecting at least one liquid fraction enriched in the at least one cannabinoid compound and the one or more additional compounds and depleted in the further compounds, said at least one liquid fraction providing the initial mixture,
the one or more additional compounds and/or the one or more further compounds comprising pesticides, plant growth regulators or combinations thereof.