US 11,754,574 B2
Red cell diluent with EDTA and methods for making and using the same
Mortimer Alzona, Norcross, GA (US); Bryan Marshall, Norcross, GA (US); Elizabeth Cope, Norcross, GA (US); Margot Borgel, Norcross, GA (US); and Teresa Welch, Norcross, GA (US)
Assigned to IMMUCOR, INC., Norcross, GA (US)
Appl. No. 16/500,713
Filed by Immucor, Inc., Norcross, GA (US)
PCT Filed Apr. 3, 2018, PCT No. PCT/US2018/025918
§ 371(c)(1), (2) Date Oct. 3, 2019,
PCT Pub. No. WO2018/187348, PCT Pub. Date Oct. 11, 2018.
Claims priority of provisional application 62/480,879, filed on Apr. 3, 2017.
Prior Publication US 2020/0191806 A1, Jun. 18, 2020
Int. Cl. G01N 33/80 (2006.01); G01N 1/38 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/80 (2013.01) [G01N 1/38 (2013.01); G01N 33/5005 (2013.01); G01N 33/6854 (2013.01)] 10 Claims
OG exemplary drawing
1. A method of preparing a well comprising:
preparing a RBC solution by suspending packed red blood cells (RBCs) in a solution comprising ethylenediaminetetraacetic acid (EDTA); and
applying the RBC solution to a well to allow the RBCs in the RBC solution to attach to the surface of the well, thereby immobilizing RBCs in the well, and
treating the immobilized RBCs with a lysing agent such that fragments of the RBCs remain immobilized in the well.