US 11,754,563 B2
Porous membranes with a polymer grafting, methods and uses thereof
Bing Li, Clifton Park, NY (US); Brian Christopher Bales, Niskayuna, NY (US); David Roger Moore, Rexford, NY (US); Jason Michael Nichols, Schenectady, NY (US); Cathryn Ellen Olsen, Wilton, NY (US); William Christopher Alberts, Saratoga Springs, NY (US); and Frank John Mondello, Niskayuna, NY (US)
Assigned to GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD, Sheffield (GB)
Filed by Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
Filed on Nov. 20, 2014, as Appl. No. 14/548,383.
Prior Publication US 2016/0146802 A1, May 26, 2016
Int. Cl. G01N 33/543 (2006.01); G01N 33/548 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 33/548 (2013.01); G01N 33/54353 (2013.01)] 13 Claims
 
1. A lateral flow immunoassay device comprising:
a sample application zone at one end of the lateral flow immunoassay device for applying a biological sample comprising a target analyte; and
a detection zone present at another end of the lateral flow immunoassay device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first antibodies immobilized on a porous nitrocellulose membrane modified via a polymer covalently grafted on the porous nitrocellulose membrane, wherein the polymer is not a copolymer, and comprises a structural unit having a formula:

OG Complex Work Unit Chemistry
wherein (i) R1 is H or CH3, (ii) x is a number of repeat units and x is at least 2, (iii) a grafting level of the

OG Complex Work Unit Chemistry
moiety is at least 300 μmol per gram of the porous nitrocellulose membrane, and (iv) B is:

OG Complex Work Unit Chemistry
and a grafting level for the

OG Complex Work Unit Chemistry
moiety of the polymer in the porous nitrocellulose membrane is less than 350 μmol of the

OG Complex Work Unit Chemistry
moiety per gram of the porous nitrocellulose membrane; and
wherein the lateral flow immunoassay device is configured to flow the biological sample along a length of the lateral flow immunoassay device from the sample application zone to the detection zone.