US 12,411,146 B2
Method for measuring amyloid β peptide
Takuya Iino, Kobe (JP); Shunsuke Watanabe, Kobe (JP); and Kouzou Suto, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Jun. 24, 2021, as Appl. No. 17/357,209.
Claims priority of application No. 2020-109825 (JP), filed on Jun. 25, 2020.
Prior Publication US 2021/0405068 A1, Dec. 30, 2021
Int. Cl. G01N 33/68 (2006.01)
CPC G01N 33/6896 (2013.01) [G01N 2800/2821 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for measuring an Aβ peptide, comprising:
mixing i) a blood sample comprising an Aβ peptide and ii) an antibody that specifically binds to the Aβ peptide, to form a complex of the Aβ peptide and the antibody;
releasing the Aβ peptide from the complex with a basic solution comprising acetonitrile and ammonia; and
measuring a released Aβ peptide in the basic solution by liquid chromatography-mass spectrometry,
wherein the basic solution containing the released Aβ peptide is used as is for liquid chromatography-mass spectrometry, and
wherein the basic solution has a pH of 11.4 or more.