US 12,411,145 B2
Methods for quantifying frataxin activity
Joan David Bettoun, Elkins Park, PA (US)
Assigned to Larimar Therapeutics, Inc., Bala Cynwyd, PA (US)
Filed by Larimar Therapeutics, Inc., Bala Cynwyd, PA (US)
Filed on Apr. 17, 2024, as Appl. No. 18/637,707.
Application 18/637,707 is a continuation of application No. 17/105,149, filed on Nov. 25, 2020, granted, now 11,988,675.
Claims priority of provisional application 62/940,214, filed on Nov. 25, 2019.
Prior Publication US 2025/0085297 A1, Mar. 13, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); G01N 33/53 (2006.01)
CPC G01N 33/6893 (2013.01) [G01N 33/5306 (2013.01); G01N 2333/902 (2013.01)] 34 Claims
 
1. A method for measuring enzymatic activity of a frataxin (FXN) protein, wherein said enzymatic activity comprises enzymatically catalyzed generation of reactive oxygen species (ROS), said method comprising:
(a) combining said FXN protein with a reducing agent and an ROS detector compound, thereby producing an assay mixture;
(b) measuring the amount of ROS generated in the assay mixture over time; and
(c) determining specific activity associated with the ROS generating activity of the FXN protein.