US 12,411,131 B2
Assay methods
Anahit Aghvanyan, Gaithersburg, MD (US); Eli N. Glezer, Del Mar, CA (US); John Kenten, Boyds, MD (US); George Sigal, Rockville, MD (US); Martin Stengelin, Gaithersburg, MD (US); and David Routenberg, Gaithersburg, MD (US)
Assigned to MESO SCALE TECHNOLOGIES, LLC., Rockville, MD (US)
Filed by MESO SCALE TECHNOLOGIES, LLC., Rockville, MD (US)
Filed on Jul. 21, 2022, as Appl. No. 17/813,965.
Application 17/813,965 is a division of application No. 16/564,208, filed on Sep. 9, 2019, granted, now 11,525,825.
Application 16/564,208 is a division of application No. 15/311,309, granted, now 10,408,823, issued on Sep. 1, 2019, previously published as PCT/US2015/030925, filed on May 15, 2015.
Claims priority of provisional application 62/055,093, filed on Sep. 25, 2014.
Claims priority of provisional application 62/049,520, filed on Sep. 12, 2014.
Claims priority of provisional application 62/048,489, filed on Sep. 10, 2014.
Claims priority of provisional application 62/013,823, filed on Jun. 18, 2014.
Claims priority of provisional application 61/993,581, filed on May 15, 2014.
Prior Publication US 2022/0357318 A1, Nov. 10, 2022
Int. Cl. C12Q 1/6832 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6844 (2018.01); G01N 33/543 (2006.01)
CPC G01N 33/54306 (2013.01) [C12Q 1/6832 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01); C12Q 2525/197 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method of detecting an analyte of interest in a sample, comprising:
(a) forming a complex on a surface, wherein the complex comprises (i) a capture reagent for the analyte that is bound to the surface; (ii) the analyte; and (iii) a detection reagent for the analyte that is linked to a nucleic acid probe, wherein the surface further comprises an anchoring reagent comprising an anchoring oligonucleotide;
(b) extending the nucleic acid probe to form an extended sequence comprising an anchoring oligonucleotide complement that is complementary to the anchoring oligonucleotide;
(c) hybridizing the anchoring oligonucleotide to the anchoring oligonucleotide complement; and
(d) measuring the amount of extended sequence bound to the surface, thereby detecting the analyte of interest in the sample.