US 12,411,063 B2
Apparatus and method for automated microdissection of tissue from slides to optimize tissue harvest from regions of interest
Manish Shiralkar, Pune (IN); Jithin Prems, Dooravani Nagar (IN); Raghubansh Bahadur Gupta, Bangalore (IN); Prasanth Perugupalli, Cary, NC (US); and Jaya Jain, Bhopal (IN)
Assigned to Pramana, Inc., Cambridge, MA (US)
Filed by Pramana, Inc., Cambridge, MA (US)
Filed on May 1, 2024, as Appl. No. 18/652,236.
Claims priority of provisional application 63/499,432, filed on May 1, 2023.
Prior Publication US 2024/0426716 A1, Dec. 26, 2024
Int. Cl. G01N 1/28 (2006.01); G01N 35/00 (2006.01); G06T 7/00 (2017.01); G06T 7/10 (2017.01)
CPC G01N 1/286 (2013.01) [G01N 35/00029 (2013.01); G06T 7/0012 (2013.01); G06T 7/10 (2017.01); G06T 2207/20084 (2013.01); G06T 2207/30024 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An apparatus for automated microdissection of tissue from slides to optimize tissue harvest from regions of interest, the apparatus comprising:
at least a processor; and
a memory communicatively connected to the at least a processor, wherein the memory contains instructions configuring the at least a processor to:
receive a stained input slide;
identify a region of interest on the stained input slide, wherein identifying the region of interest comprises using a registration module to:
log a sample size from the stained input slide;
document a plurality of slides sectioned for analysis;
predict a size and a location of the region of interest as a function of the sample size and the number of slides; and
register the identified region of interest across a plurality of unstained slides;
generate a segmentation map of the region of interest as a function of a segmentation algorithm; and
register a segmented region of interest, as a function of the segmentation map, onto an unstained slide, wherein registering the segmented region of interest onto the unstained slide comprises:
determining an orientation of the unstained slide corresponding to the segmented region of interest of the stained input slide;
recording the orientation of the unstained slide relative to a reference plane; and
registering the segmented region of interest to the unstained slide.