	US 12,410,488 B2
	Method for detecting coronavirus (SARS-CoV-2)
Kota Yokono, Tochigi (JP); Machiko Yamamoto, Tochigi (JP); Syota Yuki, Tochigi (JP); Ai Fukaya, Tochigi (JP); and Yutaka Kubota, Tochigi (JP)
Assigned to EIKEN KAGAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 17/905,497
Filed by EIKEN KAGAKU KABUSHIKI KAISHA, Tokyo (JP)
PCT Filed Mar. 3, 2021, PCT No. PCT/JP2021/008261 § 371(c)(1), (2) Date Sep. 1, 2022, PCT Pub. No. WO2021/177372, PCT Pub. Date Sep. 10, 2021.
Claims priority of application No. 2020-038195 (JP), filed on Mar. 5, 2020; and application No. 2020-191630 (JP), filed on Nov. 18, 2020.
Prior Publication US 2023/0125553 A1, Apr. 27, 2023
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6888 (2018.01); C12Q 1/70 (2006.01)

CPC C12Q 1/701 (2013.01) [C12Q 1/6844 (2013.01); C12Q 1/6888 (2013.01); C12Q 2600/16 (2013.01)]

16 Claims

1. A primer set for detecting SARS-CoV-2 virus through amplification by a LAMP method,

wherein the primer set consists of at least one selected from the group consisting of (c1) and (d1):

(c1) a primer set consisting of an F3 primer consisting of SEQ ID NO: 14, a B3 primer consisting of SEQ ID NO: 15, an FIP primer consisting of SEQ ID NO: 16, and a BIP primer consisting of SEQ ID NO: 17; and

(d1) a primer set consisting of an F3 primer consisting of SEQ ID NO: 21, a B3 primer consisting of SEQ ID NO: 22, a B4 primer consisting of SEQ ID NO: 23, an FIP primer consisting of SEQ ID NO: 24, and a BIP primer consisting of SEQ ID NO: 25.