	US 12,410,485 B2
	Non-invasive assays for embryo quality
Hasan Khatib, Fitchburg, WI (US)
Assigned to Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Feb. 10, 2022, as Appl. No. 17/668,553.
Application 17/668,553 is a division of application No. 16/278,723, filed on Feb. 19, 2019, granted, now 11,261,500.
Application 16/278,723 is a division of application No. 15/155,068, filed on May 15, 2016, granted, now 10,253,374, issued on Apr. 9, 2019.
Claims priority of provisional application 62/161,894, filed on May 15, 2015.
Prior Publication US 2023/0131551 A1, Apr. 27, 2023
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6888 (2018.01)

CPC C12Q 1/6888 (2013.01) [C12Q 2600/124 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01)]

6 Claims

1. A method for selectively implanting a bovine embryo cultured in a culture medium into a female bovine animal for further development, the method comprising:

a) extracting RNA from the culture medium containing the bovine embryo;

b) detecting an expression level of a miRNA bta-miR-148a-having SEQ ID NO: 10 in the extracted RNA;

c) comparing the expression level of the miRNA in the culture medium to (i) an expression level of the miRNA in a culture medium containing degenerative embryos or (ii) a previously established standard value of the expression level of the miRNA, and detecting that the expression level is decreased in the miRNA from the culture medium of step (a) and selecting an embryo whose expression level of the miRNA bta-miR-148a (SEQ ID NO: 10) is decreased; and

d) transferring the embryo into a female bovine for further development, whereby the transferred embryo has a higher likelihood of successful development compared to an embryo not subjected to the selection.