US 12,410,467 B2
Bisulfite-free, whole genome methylation analysis
Chunxiao Song, Oxford (GB); Jingfei Cheng, Oxford (GB); Paulina Siejka-Zielinska, Oxford (GB); and Yibin Liu, Oxford (GB)
Assigned to LUDWIG INSTITUTE FOR CANCER RESEARCH LTD, Zurich (CH)
Appl. No. 17/625,500
Filed by LUDWIG INSTITUTE FOR CANCER RESEARCH LTD, Zurich (CH)
PCT Filed Jul. 8, 2020, PCT No. PCT/IB2020/056435
§ 371(c)(1), (2) Date Jan. 7, 2022,
PCT Pub. No. WO2021/005537, PCT Pub. Date Jan. 14, 2021.
Claims priority of provisional application 62/871,444, filed on Jul. 8, 2019.
Prior Publication US 2022/0275424 A1, Sep. 1, 2022
Int. Cl. C12Q 1/683 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/683 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12Q 2600/154 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for cleaving a modified target DNA, the method comprising:
converting 5-carboxylcytosine (5caC) and/or 5-formylcytosine (5fC) in a target DNA to dihydrouracil (DHU) with a borane reducing agent to provide a modified target DNA comprising one or more DHU; and
contacting the modified target DNA comprising one or more DHU with one or more DHU-sensitive endonucleases that cleave the modified target DNA at, or adjacent to, the one or more DHU.