| CPC C12Q 1/22 (2013.01) [C12M 37/06 (2013.01); G01N 21/64 (2013.01); G01N 21/94 (2013.01)] | 27 Claims |

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1. A biological indicator (BI) reader configured to determine the presence of viable spores in at least one biological indicator, the biological indicator reader comprising:
a reader housing comprising a panel assembly comprising at least one BI opening, the BI opening being configured to receive the biological indicator therethrough;
at least one BI bay configured to receive the biological indicator, and the at least one BI bay comprising a BI bay window;
a heater, the heater being configured to heat the at least one BI bay;
a germinant activator;
an optical assembly comprising:
an excitation source configured to emit light through the BI bay window of the BI bay; and
a camera assembly comprising a camera configured to capture images passing through the BI bay window; and
a controller configured to control the optical assembly and to collect information relating to the images collected by the camera.
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