US 12,410,456 B2
Cell-free glycoprotein synthesis (CFGPS) in prokaryotic cell lysates enriched with components for glycosylation
Michael Christopher Jewett, Evanston, IL (US); Jessica Carol Stark, Evanston, IL (US); Matthew P. DeLisa, Ithaca, NY (US); and Thapakorn Jaroentomeechai, Ithaca, NY (US)
Assigned to Northwestern University, Evanston, IL (US); and Cornell University, Ithaca, NY (US)
Filed by Northwestern University, Evanston, IL (US); and Cornell University, Ithaca, NY (US)
Filed on Jul. 6, 2022, as Appl. No. 17/811,051.
Application 17/811,051 is a division of application No. 16/023,134, filed on Jun. 29, 2018, granted, now 11,453,901.
Application 16/023,134 is a continuation in part of application No. PCT/US2016/069512, filed on Dec. 30, 2016.
Claims priority of provisional application 62/273,124, filed on Dec. 30, 2015.
Prior Publication US 2023/0159972 A1, May 25, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/52 (2006.01); C07K 14/47 (2006.01); C12N 1/20 (2006.01); C12N 15/62 (2006.01); C12P 19/18 (2006.01); C12P 21/00 (2006.01)
CPC C12P 21/005 (2013.01) [C07K 14/473 (2013.01); C12N 1/20 (2013.01); C12N 15/52 (2013.01); C12N 15/62 (2013.01); C12P 19/18 (2013.01); C12N 2330/50 (2013.01)] 17 Claims
 
1. A cell-free platform for performing glycoprotein synthesis of a target protein, the platform comprising a cell-free lysate from a genetically modified strain of Escherichia coli (E. coli) bacteria comprising:
a heterologous oligosaccharyltransferase (OST) gene which is expressed in the modified strain of E. coli bacteria and which encodes an OST enzyme, wherein the OST enzyme is a component of the cell-free lysate; and wherein the lysate does not comprise the target protein.