	US 12,410,452 B2
	Method for producing lysine from microorganism and for constructing microorganism
Jibin Sun, Tianjin (CN); Jiuzhou Chen, Tianjin (CN); Ping Zheng, Tianjin (CN); Wenjuan Zhou, Tianjin (CN); Xuan Guo, Tianjin (CN); Tuo Shi, Tianjin (CN); Jiao Liu, Tianjin (CN); and Yanhe Ma, Tianjin (CN)
Assigned to Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin (CN)
Appl. No. 17/758,770
Filed by Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin (CN)
PCT Filed Jan. 13, 2021, PCT No. PCT/CN2021/071517 § 371(c)(1), (2) Date Jul. 13, 2022, PCT Pub. No. WO2021/143727, PCT Pub. Date Jul. 22, 2021.
Claims priority of application No. 202010042203.2 (CN), filed on Jan. 15, 2020.
Prior Publication US 2023/0242952 A1, Aug. 3, 2023
Int. Cl. C12P 13/08 (2006.01); C07K 14/34 (2006.01); C12N 9/06 (2006.01); C12N 9/12 (2006.01)

CPC C12P 13/08 (2013.01) [C07K 14/34 (2013.01); C12N 9/0016 (2013.01); C12N 9/1217 (2013.01); C12Y 104/01016 (2013.01); C12Y 207/02004 (2013.01)]

14 Claims

1. An L-lysine producing bacterium Corynebacterium glutamicum, wherein, compared to a wild-type bacterium containing an endogenous polypeptide, the L-lysine producing bacterium has at least one of the following characteristics (i) to (iii):

(i) a reduced or eliminated polypeptide activity of the endogenous polypeptide;

(ii) a reduced or eliminated expression level of a coding gene of the endogenous polypeptide; and

(iii) a reduced or eliminated expression level of a polynucleotide of interest;

wherein by having at least one of the characteristics (i) to (iii), L-lysine production in the L-lysine producing bacterium Corynebacterium glutamicum is increased as compared to that in the wild-type bacterium,

wherein the endogenous polypeptide is a polypeptide having the amino acid sequence of SEQ ID NO: 1, or a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1; and

the polynucleotide of interest is a polynucleotide having the nucleotide sequence of SEQ ID NO: 2, or a polynucleotide having at least 90% sequence identity to the nucleotide sequence of SEQ ID NO: 2.