	US 12,410,449 B2
	Endonuclease systems
Brian C. Thomas, Berkeley, CA (US); Christopher Brown, Albany, CA (US); Cristina Butterfield, Oakland, CA (US); Lisa Alexander, Albany, CA (US); Daniela S. A. Goltsman, Oakland, CA (US); Rebecca Lamothe, Albany, CA (US); and Isabel Nocedal, Oakland, CA (US)
Assigned to Metagenomi, Inc., Emeryville, CA (US)
Filed by Metagenomi, Inc., Emeryville, CA (US)
Filed on May 21, 2024, as Appl. No. 18/669,712.
Application 18/669,712 is a continuation of application No. PCT/US2022/080437, filed on Nov. 23, 2022.
Claims priority of provisional application 63/356,908, filed on Jun. 29, 2022.
Claims priority of provisional application 63/289,981, filed on Dec. 15, 2021.
Claims priority of provisional application 63/282,999, filed on Nov. 24, 2021.
Prior Publication US 2024/0294948 A1, Sep. 5, 2024
Int. Cl. C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01)

CPC C12N 15/907 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 2310/20 (2017.05); C12N 2310/531 (2013.01); C12N 2800/80 (2013.01)]

29 Claims

1. An engineered nuclease system comprising:

(a) an endonuclease or a nucleic acid encoding said endonuclease, wherein said endonuclease comprises a RuvC domain and an HNH domain, wherein said endonuclease comprises a sequence having at least 85% sequence identity to SEQ ID NO: 1316; and

(b) an engineered guide ribonucleic acid structure or a nucleic acid encoding said engineered guide ribonucleic acid structure, wherein said engineered guide ribonucleic acid structure is configured to form a complex with said endonuclease, wherein said engineered guide ribonucleic acid structure comprises:

(i) a guide ribonucleic acid sequence configured to hybridize to a target nucleic acid sequence; and

(ii) a tracr ribonucleic acid sequence configured to bind to said endonuclease.