	US 12,410,433 B2
	Building designer RNA nano-structures for synthetic biology applications
Laising Yen, Pearland, TX (US)
Assigned to Baylor College of Medicine, Houston, TX (US)
Filed by Baylor College of Medicine, Houston, TX (US)
Filed on Mar. 1, 2023, as Appl. No. 18/176,868.
Application 18/176,868 is a continuation of application No. 16/348,445, granted, now 11,597,935, issued on Mar. 7, 2023, previously published as PCT/US2017/061186, filed on Nov. 10, 2017.
Claims priority of provisional application 62/420,085, filed on Nov. 10, 2016.
Prior Publication US 2023/0332161 A1, Oct. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/115 (2010.01); C07H 21/02 (2006.01); C12N 15/10 (2006.01); C40B 40/06 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01)

CPC C12N 15/115 (2013.01) [C07H 21/02 (2013.01); C12N 15/1093 (2013.01); C40B 40/06 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12N 2310/51 (2013.01); C12N 2310/531 (2013.01)]

7 Claims

1. A method of producing a structure in a cell, comprising:

introducing an expression unit into a cell,

wherein the expression unit comprises a nucleic acid sequence encoding RNA units and a nucleic acid sequence encoding a CRISPR system;

wherein the RNA units comprise:

at least one three-way junction;

at least one kissing loop; and

a single stranded region;

wherein the RNA units self-assemble to form the structure,

wherein the structure comprises at least two RNA units configured in a pattern of one, two, or three dimensions,

wherein the at least two RNA units are bound to each other through complementary binding of a kissing loop on each RNA unit,

wherein when the loops are kissing between separate RNA units, the arms of the respective kissing loops from the separate RNA units are at a substantially 180 degree angle.