	US 12,410,432 B2
	L-RNA aptamer cyclization and use thereof
Chun Kit Kwok, Kowloon (HK); and Danyang Ji, Kowloon (HK)
Assigned to CITY UNIVERSITY OF HONG KONG, Kowloon (HK)
Filed by City University, Kowloon (HK)
Filed on Apr. 21, 2021, as Appl. No. 17/236,600.
Prior Publication US 2022/0348922 A1, Nov. 3, 2022
Int. Cl. C12N 15/115 (2010.01); C12Q 1/68 (2018.01)

CPC C12N 15/115 (2013.01) [C12N 2310/16 (2013.01)]

7 Claims

1. A method of linking two terminal ends of oligonucleotide, wherein one of the terminal oligonucleotide ends has a 5′ terminal alkyne; and the other one of the terminal oligonucleotide ends has a 3′ azide residue; comprising the step of:

linking the 5′ terminal alkyne to the 3′ azide residue,

wherein the oligonucleotide is L-Apt.4-1c comprising the nucleotide sequence of SEQ ID NO: 1.