US 12,410,427 B2
System and method for automated single cell processing
Kalyan Handique, Hercules, CA (US); Austin Payne, Hercules, CA (US); Sida Wang, Hercules, CA (US); Patrick Michael Tegels, Hercules, CA (US); William T. Koederitz, Hercules, CA (US); Daniel Genord, Hercules, CA (US); Grey Parker, Hercules, CA (US); Brian Boniface, Hercules, CA (US); Katlyn Curtin Mehne, Hercules, CA (US); and Alec William Hitchiner, Hercules, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Jan. 6, 2023, as Appl. No. 18/094,278.
Application 18/094,278 is a continuation of application No. 16/867,256, filed on May 5, 2020, granted, now 11,578,322.
Claims priority of provisional application 62/844,470, filed on May 7, 2019.
Claims priority of provisional application 62/866,726, filed on Jun. 26, 2019.
Prior Publication US 2023/0159917 A1, May 25, 2023
Int. Cl. B01L 3/00 (2006.01); B01L 7/00 (2006.01); B01L 9/02 (2006.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01); C12Q 1/6844 (2018.01); G01F 23/292 (2006.01); G01N 35/00 (2006.01)
CPC C12N 15/1096 (2013.01) [B01L 3/502 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); B01L 3/52 (2013.01); B01L 7/52 (2013.01); B01L 9/02 (2013.01); C12N 1/06 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6844 (2013.01); G01F 23/292 (2013.01); G01N 35/00 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/16 (2013.01); B01L 2300/041 (2013.01); B01L 2300/042 (2013.01); B01L 2300/047 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/123 (2013.01); B01L 2300/16 (2013.01); B01L 2300/1805 (2013.01); B01L 2300/1894 (2013.01); B01L 2400/043 (2013.01); B01L 2400/06 (2013.01); B01L 2400/0633 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system for processing a sample, the system comprising:
a reagent cartridge;
a deck configured to support and position:
a sample processing cartridge comprising a set of wells,
a lid configured to cover an access region over the set of wells of the sample processing cartridge, the lid comprising an open mode in which the access region is uncovered and a closed mode in which the access region is covered, the lid further comprising a releasing element; and a gantry coupled to the deck and comprising:
a set of tracks defining paths of movement along a set of axes, within a three dimensional volume bound by a first side of the deck, for a pipette interface;
a heating and cooling subsystem comprising a thermal body configured to thermally interface with the sample processing cartridge; and
a lid opening tool configured to transition the lid between the open mode and the closed mode,
with retention of the sample processing cartridge thermally interfaced with the heating and cooling subsystem, wherein the lid-opening tool comprises a linking element configured to engage the releasing element on the lid.
 
11. A method comprising:
retaining a sample processing cartridge at a deck;
wherein the sample processing cartridge comprises:
a set of wells, and
a lid configured to cover an access region over the set of wells of the
sample processing cartridge, the lid comprising an open position in which the access region is uncovered and a closed position in which the access region is covered, the lid further comprising a releasing element;
transferring a sample to the set of wells of the sample processing cartridge;
performing a set of operations in coordination with transferring a set of reagents from a reagent cartridge to the set of wells of the sample processing cartridge, wherein the set of operations comprises:
priming the sample processing cartridge upon dispensing a priming solution into the sample processing cartridge;
performing a reverse transcription operation, at the set of wells of the sample processing cartridge, on nucleic acids of the sample;
performing a strand denaturing and second strand synthesis operation upon performance of the reverse transcription operation;
transitioning, using a lid-opening tool the lid between the open position and the closed position, with
retention of the sample processing cartridge at the deck, wherein the lid-opening tool comprises a linking element configured to engage the releasing element on the lid; and
performing a cDNA amplification operation upon performance of the strand denaturing and second strand synthesis operation.