	US 12,410,420 B2
	Compositions and methods comprising variant microbial proteases
Joshua R. Basler, Palo Alto, CA (US); Luis G. Cascao-Pereira, Redwood City, CA (US); David A. Estell, San Francisco, CA (US); and James T. Kellis, Jr., Palo Alto, CA (US)
Assigned to Danisco US Inc., Palo Alto, CA (US)
Filed by DANISCO US INC., Palo Alto, CA (US)
Filed on Oct. 14, 2021, as Appl. No. 17/501,013.
Application 14/537,761 is a division of application No. 12/996,564, abandoned, previously published as PCT/US2009/046066, filed on Jun. 3, 2009.
Application 17/501,013 is a continuation of application No. 15/801,552, filed on Nov. 2, 2017, abandoned.
Application 15/801,552 is a continuation of application No. 14/577,443, filed on Dec. 19, 2014, abandoned.
Application 14/577,443 is a continuation of application No. 14/537,761, filed on Nov. 10, 2014, granted, now 10,563,189.
Claims priority of provisional application 61/059,695, filed on Jun. 6, 2008.
Prior Publication US 2022/0025350 A1, Jan. 27, 2022
Int. Cl. C12N 9/54 (2006.01); A23K 20/189 (2016.01); C11D 3/386 (2006.01); C12N 9/52 (2006.01)

CPC C12N 9/54 (2013.01) [A23K 20/189 (2016.05); C11D 3/386 (2013.01); C11D 3/38681 (2013.01); C12N 9/52 (2013.01); C11D 2111/12 (2024.01); C12Y 304/21062 (2013.01)]

8 Claims

1. An isolated subtilisin variant comprising a set of substitutions comprising S53G-S78N wherein the subtilisin variant has at least 90% amino acid sequence identity with the amino acid sequence of SEQ ID NO: 1, and wherein said substitutions are at positions equivalent to the positions of BPN′ subtilisin set forth in SEQ ID NO:1.