US 12,410,414 B2
Process of extracting oil from thin stillage
Michael John Akerman, Wake Forest, NC (US); Nathaniel Edward Kreel, Louisburg, NC (US); Melissa Carrie Hooss, Franklinton, NC (US); and Xinyu Shen, Wake Forest, NC (US)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Feb. 10, 2023, as Appl. No. 18/167,707.
Application 18/167,707 is a continuation of application No. 16/062,769, granted, now 11,781,123, previously published as PCT/US2016/067154, filed on Dec. 16, 2016.
Claims priority of provisional application 62/430,695, filed on Dec. 6, 2016.
Claims priority of provisional application 62/324,107, filed on Apr. 18, 2016.
Claims priority of provisional application 62/271,182, filed on Dec. 22, 2015.
Claims priority of provisional application 62/271,063, filed on Dec. 22, 2015.
Prior Publication US 2023/0303986 A1, Sep. 28, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/14 (2006.01); C12N 9/16 (2006.01); C12N 9/20 (2006.01); C12N 9/26 (2006.01); C12N 9/28 (2006.01); C12N 9/34 (2006.01); C12N 9/42 (2006.01); C12N 9/44 (2006.01); C12N 9/50 (2006.01); C12P 7/06 (2006.01); C12P 19/14 (2006.01); C12P 7/64 (2022.01)
CPC C12N 9/14 (2013.01) [C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12N 9/2414 (2013.01); C12N 9/2417 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2457 (2013.01); C12N 9/50 (2013.01); C12P 7/06 (2013.01); C12P 19/14 (2013.01); C12Y 301/03008 (2013.01); C12Y 301/04 (2013.01); C12Y 301/04011 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01041 (2013.01); C12Y 304/21062 (2013.01); C12Y 304/23023 (2013.01); C12Y 304/24039 (2013.01); C12P 7/64 (2013.01); Y02E 50/10 (2013.01)] 20 Claims
 
1. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins with an alpha-amylase at a temperature above the initial gelatinization temperature;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) optionally concentrating the thin stillage into syrup; and
(g) recovering oil from the thin stillage and/or optionally the syrup,
wherein a phospholipase C having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 is present and/or added during steps (b) and/or (c).