US 12,410,411 B2
Biocatalytic techniques
Jonathan Charles Paul Steele, Abingdon (GB); Antonio De Riso, Abingdon (GB); Francesco Falcioni, Manchester (GB); Stephen Keith Wrigley, Abingdon (GB); Emily Jade Hopkins, Abingdon (GB); Aksana Rimu Khan, Abingdon (GB); Kinga Linda Nytko, Abingdon (GB); Vincent Poon, Abingdon (GB); John Maxim Ward, London (GB); and Sebastian Schulz, Schenkendöbern (DE)
Assigned to HYPHA DISCOVERY LIMITED, Abingdon (GB)
Appl. No. 17/778,315
Filed by HYPHA DISCOVERY LIMITED, Abingdon (GB)
PCT Filed Nov. 23, 2020, PCT No. PCT/GB2020/052982
§ 371(c)(1), (2) Date May 19, 2022,
PCT Pub. No. WO2021/099807, PCT Pub. Date May 27, 2021.
Claims priority of application No. 1917077 (GB), filed on Nov. 22, 2019.
Prior Publication US 2023/0040326 A1, Feb. 9, 2023
Int. Cl. C12N 9/02 (2006.01); C12N 15/70 (2006.01); C12P 7/26 (2006.01); C12P 7/6409 (2022.01); C12P 13/00 (2006.01); C12P 13/02 (2006.01); C12P 17/04 (2006.01); C12P 17/10 (2006.01); C12P 17/12 (2006.01); C12P 17/16 (2006.01); C12P 17/18 (2006.01)
CPC C12N 9/0071 (2013.01) [C12N 15/70 (2013.01); C12P 7/26 (2013.01); C12P 7/6409 (2013.01); C12P 13/001 (2013.01); C12P 13/02 (2013.01); C12P 17/04 (2013.01); C12P 17/10 (2013.01); C12P 17/12 (2013.01); C12P 17/16 (2013.01); C12P 17/165 (2013.01); C12P 17/167 (2013.01); C12P 17/181 (2013.01); C12P 17/182 (2013.01); C12Y 114/14001 (2013.01)] 13 Claims
 
1. A microorganism comprising a nucleic acid molecule comprising a nucleotide sequence encoding a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO: 3, or a variant thereof having an amino acid sequence having at least 98% identity thereto and having CYP450 activity, wherein the nucleotide sequence encoding the enzyme is heterologous to the microorganism, or a lysate of said microorganism.