| CPC C07K 14/62 (2013.01) [C07K 14/43509 (2013.01); C30B 7/02 (2013.01); C30B 29/58 (2013.01); G16B 15/00 (2019.02); A61K 38/00 (2013.01); A61P 3/10 (2018.01); C07K 2299/00 (2013.01)] | 18 Claims |
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1. An insulin analog comprising a human insulin A chain peptide at least 85% identical to wild-type human insulin A chain provided as SEQ ID NO: 24, and a human insulin B chain peptide comprising 90% identity to amino acids 1-22 of wild-type human insulin B chain provided as SEQ ID NO:25 and lacking the last 8 amino acids of the C-terminus of wild-type human insulin B chain, wherein the human insulin B chain peptide comprises a substitution at amino acid 10 and amino acid 20, wherein the substitution at amino acid 20 is G20Y, G20F, or G20P, and wherein the substitution at amino acid 10 is H10E, H10D or H10Q.
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