US 12,410,083 B2
Microbe-based systems, compositions, and methods thereof
Bruce J. Godfrey, Seattle, WA (US); Mari-Karoliina Henriikka Winkler, Seattle, WA (US); and David Stahl, Seattle, WA (US)
Assigned to University of Washington, Seattle, WA (US)
Appl. No. 17/791,676
Filed by University of Washington, Seattle, WA (US)
PCT Filed Jan. 12, 2021, PCT No. PCT/US2021/013127
§ 371(c)(1), (2) Date Jul. 8, 2022,
PCT Pub. No. WO2021/146212, PCT Pub. Date Jul. 22, 2021.
Claims priority of provisional application 62/960,494, filed on Jan. 13, 2020.
Prior Publication US 2023/0046027 A1, Feb. 16, 2023
Int. Cl. C02F 3/34 (2023.01); C02F 3/10 (2023.01); C02F 3/30 (2023.01); C12M 1/12 (2006.01); C12N 1/20 (2006.01); C12N 11/04 (2006.01)
CPC C02F 3/348 (2013.01) [C02F 3/102 (2013.01); C02F 3/307 (2013.01); C02F 3/341 (2013.01); C12M 25/10 (2013.01); C12N 1/20 (2013.01); C12N 11/04 (2013.01); C02F 3/302 (2013.01); C12M 25/02 (2013.01); Y02W 10/10 (2015.05)] 20 Claims
OG exemplary drawing
 
1. A microorganism support structure, comprising:
(a) a gas-permeable layer comprising two opposing surfaces;
(b) an adhesive, coating one surface of the layer to form an adhesive-coated surface;
(c) a first microorganism and a second microorganism disposed on the adhesive-coated surface of the layer, wherein the second microorganism is configured to attach to the adhesive-coated surface to form a second microorganism-coated surface of the layer, and the first microorganism is configured to attach to the second microorganism-coated surface such that the second microorganism decreases in population density and number as a function of distance from the gas-permeable layer, and the first microorganism increases in population density and number as a function of distance from the gas-permeable layer, wherein the first microorganism consumes a nitrite produced by the second microorganism.