US 12,409,457 B2
Devices and method for enrichment and alteration of cells and other particles
Lotien Richard Huang, Chestnut Hill, MA (US); Thomas A. Barber, Sudbury, MA (US); Bruce L. Carvalho, Watertown, MA (US); Ravi Kapur, Sharon, MA (US); Paul Vernucci, Billerica, MA (US); Mehmet Toner, Charlestown, MA (US); and Zihua Wang, Newton, MA (US)
Assigned to The General Hospital Corporation, Boston, MA (US); and GPB Scientific, LLC, Richmond, VA (US)
Filed by The General Hospital Corporation, Boston, MA (US); and GPB Scientific, LLC, Richmond, VA (US)
Filed on Sep. 15, 2020, as Appl. No. 17/021,100.
Application 14/930,313 is a division of application No. 13/232,781, filed on Sep. 14, 2011, granted, now 9,174,222, issued on Nov. 3, 2015.
Application 17/021,100 is a continuation of application No. 15/965,128, filed on Apr. 27, 2018, granted, now 10,786,817.
Application 15/965,128 is a continuation of application No. 14/930,313, filed on Nov. 2, 2015, granted, now 9,956,562, issued on May 1, 2018.
Application 13/232,781 is a continuation of application No. 11/449,149, filed on Jun. 8, 2006, granted, now 8,021,614, issued on Sep. 20, 2011.
Application 11/449,149 is a continuation of application No. PCT/US2006/012820, filed on Apr. 5, 2006.
Claims priority of provisional application 60/704,067, filed on Jul. 29, 2005.
Claims priority of provisional application 60/668,415, filed on Apr. 5, 2005.
Prior Publication US 2021/0178403 A1, Jun. 17, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B03C 1/30 (2006.01); B01L 3/00 (2006.01); B03C 1/32 (2006.01); C12M 1/00 (2006.01); C12Q 1/686 (2018.01); G01N 1/40 (2006.01); G01N 33/50 (2006.01); G01N 35/00 (2006.01); B33Y 80/00 (2015.01)
CPC B03C 1/30 (2013.01) [B01L 3/502746 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); B03C 1/32 (2013.01); C12M 47/04 (2013.01); C12M 47/06 (2013.01); C12Q 1/686 (2013.01); G01N 1/4077 (2013.01); G01N 33/5044 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0472 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); B03C 2201/18 (2013.01); B33Y 80/00 (2014.12); G01N 1/40 (2013.01); G01N 2035/00237 (2013.01); Y10T 137/0318 (2015.04); Y10T 137/8593 (2015.04); Y10T 436/25 (2015.01); Y10T 436/25375 (2015.01)] 27 Claims
 
1. A method of enriching a sample in fetal cells relative to maternal cells, the method comprising:
enriching a mixture of maternal and fetal cells for fetal cells relative to maternal cells to produce an enriched sample;
performing lysis of all types of cells in the enriched sample to lyse both maternal cells and fetal cells in the enriched sample to release maternal nuclei and fetal nuclei;
depositing the maternal nuclei and fetal nuclei onto a solid support;
identifying the fetal nuclei; and
performing genetic analysis of fetal DNA from the fetal nuclei.