US 12,409,449 B2
Flow cells
Yir-Shyuan Wu, Albany, CA (US); Tarun Kumar Khurana, Fremont, CA (US); Yasaman Farshchi, San Francisco, CA (US); Xi-Jun Chen, San Carlos, CA (US); and Bernard Hirschbein, San Francisco, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Appl. No. 17/420,048
Filed by ILLUMINA, INC., San Diego, CA (US)
PCT Filed Jul. 22, 2020, PCT No. PCT/US2020/043057
§ 371(c)(1), (2) Date Jun. 30, 2021,
PCT Pub. No. WO2021/021515, PCT Pub. Date Feb. 4, 2021.
Claims priority of provisional application 62/881,597, filed on Aug. 1, 2019.
Prior Publication US 2022/0080415 A1, Mar. 17, 2022
Int. Cl. B01L 3/00 (2006.01); C08F 220/56 (2006.01); C08J 9/36 (2006.01); C12Q 1/6806 (2018.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502707 (2013.01); C08F 220/56 (2013.01); C08J 9/365 (2013.01); C12Q 1/6806 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/16 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0877 (2013.01); C08J 2205/022 (2013.01); C08J 2333/26 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A flow cell, comprising:
a substrate;
a flow lane defined in the substrate;
a cationic polymeric hydrogel on the substrate in at least a portion of the flow lane, the cationic polymeric hydrogel including a cationic moiety i) integrated into a monomeric unit of an initial polymeric hydrogel or ii) attached to the monomeric unit of the initial polymeric hydrogel through a linker;
an amplification primer attached to the cationic polymeric hydrogel;
a lid or second substrate bonded to the substrate;
an input port in fluid communication with the flow lane; and
an exit port in fluid communication with the flow lane.
 
10. A method, comprising:
introducing a fluid, including a positively chargeable moiety, to a flow cell including:
a substrate;
a flow lane defined in the substrate;
an initial polymeric hydrogel on the substrate in at least a portion of the flow lane, the initial polymeric hydrogel having a surface moiety selected from the group consisting of a negatively chargeable atom, an azide group, and an alkyne group;
an amplification primer attached to the initial polymeric hydrogel;
a lid or second substrate bonded to the substrate;
an input port in fluid communication with the flow lane; and
an exit port in fluid communication with the flow lane; and
incubating the initial polymeric hydrogel in the fluid at a temperature and for a time, thereby forming a cationic polymeric hydrogel on the substrate in the at least the portion of the flow lane, the cationic polymeric hydrogel including a cationic moiety.