US 12,408,659 B2
DMSO-free cryopreservation solution and preparation method and use thereof
Jie Yan, Beijing (CN); Jie Qiao, Beijing (CN); Liying Yan, Beijing (CN); Rong Li, Beijing (CN); Jianjun Wang, Beijing (CN); Shenglin Jin, Beijing (CN); and Jianyong Lv, Beijing (CN)
Assigned to PEKING UNIVERSITY THIRD HOSPITAL, Beijing (CN); and INSTITUTE OF CHEMISTRY, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
Appl. No. 17/594,202
Filed by PEKING UNIVERSITY THIRD HOSPITAL, Beijing (CN); and INSTITUTE OF CHEMISTRY, CHINESE ACADEMY OF SCIENCES, Beijing (CN)
PCT Filed Mar. 2, 2020, PCT No. PCT/CN2020/077473
§ 371(c)(1), (2) Date Oct. 6, 2021,
PCT Pub. No. WO2020/207151, PCT Pub. Date Oct. 15, 2020.
Claims priority of application No. 201910281978.2 (CN), filed on Apr. 9, 2019; and application No. 201910281986.7 (CN), filed on Apr. 9, 2019.
Prior Publication US 2022/0167610 A1, Jun. 2, 2022
Int. Cl. A01N 1/125 (2025.01); C07K 5/062 (2006.01); C07K 5/072 (2006.01); C07K 5/078 (2006.01); C07K 5/083 (2006.01)
CPC A01N 1/125 (2025.01) [C07K 5/0606 (2013.01); C07K 5/06095 (2013.01); C07K 5/06165 (2013.01); C07K 5/0806 (2013.01); C07K 5/081 (2013.01)] 20 Claims
 
1. A DMSO-free cryopreservation solution, comprising, per 100 mL, 0.01-50 g of a biomimetic ice growth inhibition material, 5.0-45 ml of a polyalcohol, a water-soluble saccharide at 0.1-1.0 mol L−1, 0-30 mL of serum and the balance of a buffer, wherein the biomimetic ice growth inhibition material is selected from an atactic polyvinyl alcohol (PVA) having a syndiotacticity of 15%-60% and a molecular weight of from great than 10 kDa to 500 kDa, or a combination of said PVA with an amino acid biomimetic ice growth inhibition material.