US 12,080,383 B2
Automated nucleic acid repeat count calling methods
A. Scott Patterson, South San Francisco, CA (US); Imran S. Haque, South San Francisco, CA (US); Eric A. Evans, South San Francisco, CA (US); and Clement Chu, South San Francisco, CA (US)
Assigned to Myriad Women's Health, Inc., South San Francisco, CA (US)
Filed by MYRIAD WOMEN'S HEALTH, INC., South San Francisco, CA (US)
Filed on Oct. 21, 2019, as Appl. No. 16/659,449.
Application 16/659,449 is a division of application No. 14/540,334, filed on Nov. 13, 2014, granted, now 10,497,463.
Claims priority of provisional application 61/904,439, filed on Nov. 14, 2013.
Claims priority of provisional application 61/903,847, filed on Nov. 13, 2013.
Prior Publication US 2020/0111543 A1, Apr. 9, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G16B 40/10 (2019.01); G16B 20/00 (2019.01); G16B 20/20 (2019.01); G16B 30/00 (2019.01)
CPC G16B 40/10 (2019.02) [G16B 20/00 (2019.02); G16B 20/20 (2019.02); G16B 30/00 (2019.02)] 21 Claims
 
1. A computer-implemented method for determining a number of nucleic acid repeats in a DNA comprising a nucleic acid repeat region, the method comprising:
a) receiving, by one or more processors, DNA size and abundance data of DNA amplification products generated from the DNA comprising the nucleic acid repeat region by using a primer set comprising a first primer recognizing the nucleic acid repeat region and a second primer recognizing a region outside of the nucleic acid repeat region;
b) generating, by the one or more processors, a set of sample data by sampling the DNA size and abundance data at a sampling frequency;
c) generating, by the one or more processors, a set of low-pass data by applying a low-pass filter to the set of sample data;
d) generating, by the one or more processors, a set of band-pass data by applying a band-pass filter to the set of sample data;
e) identifying, by the one or more processors, one or more peaks in the set of low-pass data;
f) identifying, by the one or more processors, one or more peaks in the set of band-pass data; and
g) identifying, by the one or more processors, a final peak representing a number of nucleic acid repeats in the nucleic acid repeat region based on the one or more peaks in the set of low-pass data and the one or more peaks in the set of band-pass data.