US 12,078,646 B2
Strategy enabled by a photo-cleavable surfactant for extracellular vesicle proteomics
Ying Ge, Madison, WI (US); Song Jin, Madison, WI (US); Kyle Brown, Madison, WI (US); and Kevin Buck, Madison, WI (US)
Assigned to Wisconsin Alumni Research Foundation
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Nov. 1, 2022, as Appl. No. 17/978,793.
Claims priority of provisional application 63/274,851, filed on Nov. 2, 2021.
Prior Publication US 2023/0138788 A1, May 4, 2023
Int. Cl. G01N 33/92 (2006.01); C11D 1/14 (2006.01); G01N 21/33 (2006.01); G01N 33/52 (2006.01)
CPC G01N 33/92 (2013.01) [C11D 1/143 (2013.01); G01N 21/33 (2013.01); G01N 33/52 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for analyzing one or more lipid vesicles comprising the steps of:
a) contacting the one or more lipid vesicles with a photo-cleavable anionic surfactant in a solution, thereby lysing the one or more lipid vesicles and generating a mixture of dissolved compounds comprising compounds obtained from within the one or more lipid vesicles, wherein the photo-cleavable anionic surfactant comprises: i) a hydrophilic head, ii) a hydrophobic tail, and iii) a photo-cleavable moiety covalently linking the hydrophilic head and hydrophobic tail; and
b) exposing the solution containing the photo-cleavable anionic surfactant and mixture of dissolved compounds to electromagnetic radiation, thereby decomposing the photo-cleavable moiety and generating an irradiated solution containing the mixture of dissolved compounds,
wherein the photo-cleavable anionic surfactant has the formula:

OG Complex Work Unit Chemistry
where n is an integer selected from 2 to 30, and R is any atom or molecule able to form a cation.