US 12,078,645 B2
Method for diagnosing disseminated intravascular coagulation (DIC)
Julie Helms, Strasbourg (FR); Florence Toti, Strasbourg (FR); Laure Stiel, Strasbourg (FR); Laurent Mauvieux, Mundolsheim (FR); and Ferhat Meziani, Oberhausbergen (FR)
Assigned to HÔPITAUX UNIVERSITAIRES DE STRASBOURG, Strasbourg (FR); and DIAGNOSTICA STAGO, Asnières-sur-Seine (FR)
Appl. No. 16/073,742
Filed by Hôpitaux Universitaires de Strasbourg, Strasbourg (FR); and Diagnostica Stago, Asnières-sur-Seine (FR)
PCT Filed Jan. 25, 2017, PCT No. PCT/FR2017/050158
§ 371(c)(1), (2) Date Jul. 27, 2018,
PCT Pub. No. WO2017/129895, PCT Pub. Date Aug. 3, 2017.
Claims priority of application No. 1650667 (FR), filed on Jan. 27, 2016; application No. 1650765 (FR), filed on Jan. 29, 2016; and application No. 1661823 (FR), filed on Dec. 1, 2016.
Prior Publication US 2019/0011466 A1, Jan. 10, 2019
Int. Cl. G01N 33/53 (2006.01); G01N 15/14 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); G01N 33/86 (2006.01)
CPC G01N 33/86 (2013.01) [G01N 15/14 (2013.01); G01N 33/5005 (2013.01); G01N 33/6893 (2013.01); G01N 2015/1486 (2013.01); G01N 2333/70503 (2013.01); G01N 2333/70596 (2013.01); G01N 2800/226 (2013.01); G01N 2800/26 (2013.01); G01N 2800/50 (2013.01); G01N 2800/52 (2013.01)] 16 Claims
 
1. An in-vitro process for predicting and/or detecting disseminated intravascular coagulation (DIC) in humans from a first biological sample that includes neutrophilse, wherein a measurement of fluorescence, of the neutrophils, predicts and/or detects DIC, and the measurement of fluorescence, of the neutrophils, is carried out using a DNA intercalating fluorochrome, the process comprising:
obtaining the first biological sample from a patient, the first biological sample including the neutrophilse;
applying DNA intercalating fluorochrome to the neutrophils, such that the DNA intercalating fluorochrome attaches to the neutrophils;
performing a measurement of fluorescence of the neutrophils, with DNA intercalating fluorochrome attached thereto, of the first biological sample, so as to determine a measured value;
comparing the measured value with a reference value;
based on the comparing, generating a result, and the result being that the measured value exceeds the reference value;
based on the result, generating a finding that the patient shows symptom of DIC; and
based on the finding, administering a treatment to the patient, and the treatment being an anticoagulant treatment for DIC.