CPC G01N 33/6851 (2013.01) [C07C 271/20 (2013.01); C12Q 1/6841 (2013.01); G01N 2400/00 (2013.01); G01N 2458/15 (2013.01)] | 23 Claims |
1. A method, comprising
a) providing a biological sample and a plurality of antibody probes, each of said probes reactive with a different target and conjugated to a photocleavable mass-tag, said antibody probes having the general structure of Mass Unit—Core Structure—Antibody, wherein said Core Structure has the structure:
b) contacting said biological sample with said antibody probes to effect binding of the antibody probes to the targets in said biological sample;
c) illuminating said mass-tags with light so as to photocleave at least a portion of said mass-tags from said probes prior to subjecting said mass-tags to mass spectrometry; and
d) detecting, using mass spectrometric imaging, said mass-tags, or fragments thereof, as molecular ions.
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