US 12,078,639 B2
Photocleavable mass-tags for multiplexed mass spectrometric imaging of tissues using biomolecular probes
Mark J. Lim, Reading, MA (US); Gargey Yagnik, Hopkinton, MA (US); and Kenneth J. Rothschild, Newton, MA (US)
Assigned to AmberGen, INC., Billerica, MA (US)
Filed by AmberGen, Inc., Watertown, MA (US)
Filed on Jul. 13, 2022, as Appl. No. 17/863,928.
Application 17/863,928 is a continuation of application No. 17/399,390, filed on Aug. 11, 2021, granted, now 11,906,527.
Claims priority of provisional application 63/106,990, filed on Oct. 29, 2020.
Prior Publication US 2022/0365098 A1, Nov. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C07C 271/20 (2006.01); C12Q 1/6841 (2018.01)
CPC G01N 33/6851 (2013.01) [C07C 271/20 (2013.01); C12Q 1/6841 (2013.01); G01N 2400/00 (2013.01); G01N 2458/15 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A method, comprising
a) providing a biological sample and a plurality of antibody probes, each of said probes reactive with a different target and conjugated to a photocleavable mass-tag, said antibody probes having the general structure of Mass Unit—Core Structure—Antibody, wherein said Core Structure has the structure:

OG Complex Work Unit Chemistry
b) contacting said biological sample with said antibody probes to effect binding of the antibody probes to the targets in said biological sample;
c) illuminating said mass-tags with light so as to photocleave at least a portion of said mass-tags from said probes prior to subjecting said mass-tags to mass spectrometry; and
d) detecting, using mass spectrometric imaging, said mass-tags, or fragments thereof, as molecular ions.