US 12,078,636 B2
Selective white blood cell lysis for immunoassay systems
Robert P Sambursky, Lakewood Ranch, FL (US)
Assigned to RAPID PATHOGEN SCREENING, INC., Sarasota, FL (US)
Appl. No. 17/786,245
Filed by Rapid Pathogen Screening, Inc., Sarasota, FL (US)
PCT Filed Dec. 18, 2020, PCT No. PCT/US2020/065911
§ 371(c)(1), (2) Date Jun. 16, 2022,
PCT Pub. No. WO2021/127374, PCT Pub. Date Jun. 24, 2021.
Claims priority of provisional application 62/949,958, filed on Dec. 18, 2019.
Prior Publication US 2023/0026491 A1, Jan. 26, 2023
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54388 (2021.08) 21 Claims
OG exemplary drawing
 
1. An immunoassay test strip with a first end and a second end, wherein fluid flows through capillary action downstream from the first end to the second end, the immunoassay test strip comprising:
an absorbent pad for receiving a blood sample for testing and selectively lysing at least white blood cells of the blood sample;
a blood separation pad downstream of the absorbent pad for withholding intact red blood cells or removing red heme from any lysed red blood cells of the blood sample, comprising at least one filter with a nitrocellulose pore size to withhold the intact red blood cells or the red heme, reducing red color interference caused by red heme background color on the immunoassay test strip by at least 10-20%;
a conjugate pad downstream of the blood separation pad containing biomarkers for binding to intracellular proteins of the white blood cells and extracellular proteins of the red blood cells within the blood sample; and
a membrane pad having at least one test line and control line for indicating results of the presence of intracellular proteins of the white blood cells and extracellular proteins of the red blood cells within the blood sample;
wherein the sample flows downstream from the first end to the second end from the absorbent pad, to the blood separation pad, to the conjugate pad and to the membrane pad;
wherein the results are discernable in ten minutes or less.