US 12,078,629 B2
Nanopore-matched protein shuttle for molecular characterization
Lene V. Hau, Cambridge, MA (US); Jene A. Golovchenko, Lexington, MA (US); and Min Chen, Amherst, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US); and UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed by PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US); and UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed on Nov. 30, 2022, as Appl. No. 18/072,189.
Application 18/072,189 is a continuation of application No. 16/629,640, granted, now 11,567,061, previously published as PCT/US2018/042490, filed on Jul. 17, 2018.
Claims priority of provisional application 62/533,227, filed on Jul. 17, 2017.
Prior Publication US 2023/0333084 A1, Oct. 19, 2023
Int. Cl. G01N 33/487 (2006.01); G01N 27/447 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/48721 (2013.01) [G01N 27/44791 (2013.01); G01N 33/5438 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A Cytolysin A (ClyA) nanopore sensor comprising:
a support structure separating a first fluidic chamber from a second fluidic chamber;
at least one ClyA nanopore disposed in the support structure with an inlet of the at least one ClyA nanopore fluidically connected to the first fluidic chamber and an outlet of the at least one nanopore fluidically connected to the second fluidic chamber; and
a protein shuttle which comprises an electrically charged protein molecule comprising avidin, wherein the electrically charged protein molecule has a radial extent that is at least as large as a smallest diameter of a lumen of the at least one ClyA nanopore;
wherein the protein shuttle further comprises at least one linking species, wherein the linking species is attached to the electrically charged protein molecule and is configured to link the protein shuttle to at least one target molecule;
and wherein the electrically charged protein molecule is configured to be electrically driven into the ClyA nanopore.