US 12,078,585 B2
Hyperspectral quantitative imaging cytometry system
José Mário Tenera Morgado, Outeiro de Gatos-Meda (PT); and Álvaro Rodríguez De La Gala, Palencia (ES)
Assigned to CYTOGNOS, S.L., Salamanca (ES)
Appl. No. 17/615,539
Filed by CYTOGNOS, S.L., Salamanca (ES)
PCT Filed May 29, 2020, PCT No. PCT/EP2020/064984
§ 371(c)(1), (2) Date Nov. 30, 2021,
PCT Pub. No. WO2020/239981, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 19382444 (EP), filed on May 31, 2019.
Prior Publication US 2022/0228968 A1, Jul. 21, 2022
Int. Cl. G01N 15/1433 (2024.01); G01J 3/12 (2006.01); G01J 3/28 (2006.01); G01J 3/32 (2006.01); G01N 15/10 (2024.01); G01N 15/1429 (2024.01); G01N 15/1434 (2024.01)
CPC G01N 15/1433 (2024.01) [G01J 3/32 (2013.01); G01N 15/1429 (2013.01); G01J 2003/1243 (2013.01); G01J 2003/2826 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/144 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A hyperspectral quantitative imaging cytometry system, comprising:
an observation region, comprising a sample holder configured to hold one or more solid-phase samples,
at least one radiation source configured to irradiate the observation region,
a collector configured to collect the radiation emitted through or reflected by the sample upon irradiation by the at least one radiation source, wherein the collector has a magnification factor value (M) equal to or lower than 20, and has a numerical aperture value equal to or higher than 0.25,
a multichannel filter configured to selectively filter the wavelength of the radiation collected by the collector, and
an image sensor configured to receive the filtered radiation and to generate an image that is a two-dimensional map of the sample, the image sensor comprising a two-dimensional array of radiation detectors,
wherein the system comprises a first lens and a second lens, wherein the first lens is configured to capture the image, and the second lens is configured to capture an intermediate image formed between the collector and the image sensor and project the intermediate image to the image sensor,
wherein the collector comprises a lens configured to capture multiple radiations from multiple spatial locations of the observation region simultaneously.