	US 12,077,802 B2
	*Metabolic engineering of E. coli* for the biosynthesis of cannabinoid**
Sonal R. Ayakar, Vancouver (CA); Sandip V. Pawar, Vancouver (CA); Steven J. Hallam, Vancouver (CA); Sazzad Hossain, Richmond (CA); Vikramaditya G. Yadav, Vancouver (CA); Protiva R. Roy, Vancouver (CA); and Sarvesh K. Srivastava, Vancouver (CA)
Assigned to INMED PHARMACEUTICALS INC., Vancouver (CA)
Appl. No. 16/644,484
Filed by INMED PHARMACEUTICALS INC., Vancouver (CA)
PCT Filed Sep. 5, 2018, PCT No. PCT/CA2018/051074 § 371(c)(1), (2) Date Mar. 4, 2020, PCT Pub. No. WO2019/046941, PCT Pub. Date Mar. 14, 2019.
Claims priority of provisional application 62/554,494, filed on Sep. 5, 2017.
Prior Publication US 2020/0291434 A1, Sep. 17, 2020
Int. Cl. C12P 7/42 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/88 (2006.01); C12N 15/70 (2006.01); C12P 5/00 (2006.01)

CPC C12P 7/42 (2013.01) [C12N 9/0004 (2013.01); C12N 9/1007 (2013.01); C12N 9/1241 (2013.01); C12N 9/88 (2013.01); C12N 15/70 (2013.01); C12P 5/007 (2013.01); C12Y 121/03007 (2015.07); C12Y 201/01255 (2015.07); C12Y 207/0706 (2013.01); C12Y 402/03027 (2013.01); C12N 2800/101 (2013.01)]

49 Claims

1. An expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a bifunctional ispDF enzyme, wherein the bifunctional ispDF enzyme comprises an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, and wherein the the bifunctional ispDF enzyme has 2-C-methyl-D-erythritol 4-phosphate cyticylyltransferase activity and 2-C-methyl-D -erythritol 2,4-cyclodiphosphate synthase activity.