	US 12,077,799 B2
	Chimeric terpene synthases
Christian Ridley, Acton, MA (US); Jue Wang, Seattle, WA (US); and Scott Marr, Boston, MA (US)
Assigned to Ginkgo Bioworks, Inc., Boston, MA (US)
Appl. No. 16/969,965
Filed by Ginkgo Bioworks, Inc., Boston, MA (US)
PCT Filed Feb. 14, 2019, PCT No. PCT/US2019/018122 § 371(c)(1), (2) Date Aug. 13, 2020, PCT Pub. No. WO2019/161141, PCT Pub. Date Aug. 22, 2019.
Claims priority of provisional application 62/630,640, filed on Feb. 14, 2018.
Prior Publication US 2021/0147880 A1, May 20, 2021
Int. Cl. C12P 5/00 (2006.01); C12N 9/88 (2006.01); C12P 7/02 (2006.01)

CPC C12P 5/007 (2013.01) [C12N 9/88 (2013.01); C12P 7/02 (2013.01); C12Y 402/03087 (2013.01)]

20 Claims

1. A method for producing one or more terpenes, comprising:

culturing a fungal cell that comprises a nucleic acid molecule encoding a synthetic terpene synthase, wherein:

a) the synthetic terpene synthase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO: 17, 22, or 29;

b) the synthetic terpene synthase comprises a DDxx(x)D/E motif; and

c) the fungal cell produces alpha-guaiene.