	US 12,077,796 B2
	Cell culture methods
Bassem Ben Yahia, Brussels (BE); Laetitia Malphettes, Brussels (BE); Nadine Kochanowski, Brussels (BE); Gill Renner, Slough (GB); Sandrine Durran, Slough (GB); and Andrew Jeffrey Yates, Slough (GB)
Assigned to UCB BIOPHARMA SRL, Brussels (BE)
Appl. No. 16/615,419
Filed by UCB BIOPHARMA SRL, Brussels (BE)
PCT Filed May 29, 2018, PCT No. PCT/EP2018/064102 § 371(c)(1), (2) Date Nov. 21, 2019, PCT Pub. No. WO2018/219968, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 1708655 (GB), filed on May 31, 2017.
Prior Publication US 2020/0239923 A1, Jul. 30, 2020
Int. Cl. C12P 21/02 (2006.01); C07K 1/16 (2006.01); C07K 16/00 (2006.01); C12N 5/00 (2006.01)

CPC C12P 21/02 (2013.01) [C07K 1/16 (2013.01); C07K 16/00 (2013.01); C12N 5/0018 (2013.01); C07K 2317/14 (2013.01); C07K 2317/565 (2013.01); C12N 2500/32 (2013.01); C12N 2510/02 (2013.01); C12N 2511/00 (2013.01); C12N 2523/00 (2013.01)]

20 Claims

1. A process for producing a recombinant protein comprising:

a) culturing host cells capable of producing a recombinant protein in a medium free of animal-derived products, wherein the host cells are CHO cells;

b) supplementing the culture during production phase with

cysteine or cystine in a total amount of from wt %12 wt % to 28 wt % of the expected total amount of recombinant protein produced; and

tryptophan in a total amount of from 8 wt % to 30 wt % of the expected total amount of recombinant protein produced,

c) and, optionally, recovering the recombinant protein from the cell culture medium wherein the recombinant protein is an antibody or an antigen-binding fragment thereof, wherein the recombinant protein is produced by the cells during said production phase, and wherein one or more initial experiments are performed to determine the expected total amount of recombinant protein produced.