US 12,077,795 B2
Method for biocatalytic protein-oligonucleotide conjugation
Brian Callahan, Binghamton, NY (US); and Timothy Owen, Huntington, NY (US)
Assigned to The Research Foundation for The State University of New York, Binghamton, NY (US)
Filed by The Research Foundation for The State University of New York, Binghamton, NY (US)
Filed on Aug. 10, 2020, as Appl. No. 16/989,843.
Application 16/989,843 is a division of application No. 15/782,391, filed on Oct. 12, 2017, granted, now 10,738,338, issued on Aug. 10, 2020.
Claims priority of provisional application 62/409,655, filed on Oct. 18, 2016.
Prior Publication US 2020/0377920 A1, Dec. 3, 2020
Int. Cl. C12P 21/00 (2006.01); A61K 47/54 (2017.01); A61K 47/55 (2017.01); C07K 1/107 (2006.01); C12N 9/10 (2006.01)
CPC C12P 21/00 (2013.01) [A61K 47/55 (2017.08); A61K 47/554 (2017.08); C07K 1/1077 (2013.01); C12N 9/1029 (2013.01); C12Y 201/01041 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for generating a polypeptide-nucleic acid conjugate, comprising:
providing a polypeptide comprising a C-terminal steroyl transferase activity, an N-terminal peptide, and an intervening electrophilic residue;
providing a nucleic acid substrate comprising a fused sterol or stanol ring system comprising:
i) a nucleophilic substituent on the A ring; and
ii) at least one nucleic acid, ligated to the fused sterol or stanol ring system by a linker; and
reacting the nucleic acid substrate and the polypeptide in a solution, to form a covalently linked polypeptide-nucleic acid conjugate.