	US 12,077,792 B2
	Engineered microorganisms for production of 2′fucosyllactose and l-fucose
Yong-Su Jin, Champaign, IL (US); Jingjing Liu, Urbana, IL (US); Sora Yu, Namyangju-si (KR); Eun Ju Yun, Geumcheon-gu (KR); Suryang Kwak, Savoy, IL (US); Kyoung Heon Kim, Seoul (KR); and Jaewon Lee, Savoy, IL (US)
Assigned to The Board of Trustees of the University of Illinois, Urbana, IL (US); and Korea University Research and Business Foundation, Seoul (KR)
Appl. No. 17/054,969
Filed by THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Urbana, IL (US); and KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
PCT Filed May 15, 2019, PCT No. PCT/US2019/032474 § 371(c)(1), (2) Date Nov. 12, 2020, PCT Pub. No. WO2019/222391, PCT Pub. Date Nov. 21, 2019.
Claims priority of provisional application 62/671,459, filed on May 15, 2018.
Prior Publication US 2021/0214705 A1, Jul. 15, 2021
Int. Cl. C12N 9/88 (2006.01); C07K 14/395 (2006.01); C12N 1/21 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 15/81 (2006.01); C12P 19/18 (2006.01)

CPC C12N 9/88 (2013.01) [C07K 14/395 (2013.01); C12N 9/0006 (2013.01); C12N 9/1051 (2013.01); C12N 15/81 (2013.01); C12P 19/18 (2013.01); C12Y 101/01271 (2013.01); C12Y 402/01047 (2013.01); C12N 2800/102 (2013.01)]

12 Claims

1. A recombinant yeast cell comprising heterologous polynucleotides encoding a GDP-mannose 4,6-dehydratase (Gmd) polypeptide, a GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase (WcaG) polypeptide, an oligosaccharide transporter polypeptide, a fucosyltransferase polypeptide, a α-L-fucosidase polypeptide, and a L-fucokinase/GDP-L-fucose phosphorylase (FKP) polypeptide, wherein the heterologous polynucleotides are operably linked to at least one expression control polynucleotide.