1. A recombinant family B archaeal DNA polymerase comprising an amino acid sequence that is at least 80% identical to a DNA polymerase amino acid sequence SEQ ID NO:8 or 12, wherein the recombinant DNA polymerase comprises at least two substitution mutations, wherein the first substitution mutation at the position functionally equivalent to Ala408 comprises a mutation to Tyr, and wherein the second substitution mutation at the position functionally equivalent to Ala409 comprises a mutation to Pro, and wherein the recombinant DNA polymerase preferentially incorporates a nucleotide having a blocking group at a faster rate than a nucleotide lacking the blocking group.
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