US 12,077,789 B2
Polymerases, compositions, and methods of use
Misha Golynskiy, San Diego, CA (US); Mariko Matsuura, San Diego, CA (US); Saurabh Nirantar, Singapore (SG); Hamed Tabatabaei Ghomi, Cambridge (GB); Seth McDonald, San Diego, CA (US); Ryan Craig, San Diego, CA (US); Sergio Peisajovich, San Diego, CA (US); Kyrie Johnson, San Diego, CA (US); Kay Klausing, San Diego, CA (US); Eric Murtfeldt, San Diego, CA (US); and Alexandra Exner, San Diego, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Jun. 1, 2022, as Appl. No. 17/829,866.
Claims priority of provisional application 63/233,243, filed on Aug. 14, 2021.
Prior Publication US 2023/0047225 A1, Feb. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01); C12Q 1/6874 (2018.01)
CPC C12N 9/1252 (2013.01) [C12Q 1/6874 (2013.01); C12Y 207/07007 (2013.01)] 33 Claims
 
1. A recombinant family B archaeal DNA polymerase comprising an amino acid sequence that is at least 80% identical to a DNA polymerase amino acid sequence SEQ ID NO:8 or 12, wherein the recombinant DNA polymerase comprises at least two substitution mutations, wherein the first substitution mutation at the position functionally equivalent to Ala408 comprises a mutation to Tyr, and wherein the second substitution mutation at the position functionally equivalent to Ala409 comprises a mutation to Pro, and wherein the recombinant DNA polymerase preferentially incorporates a nucleotide having a blocking group at a faster rate than a nucleotide lacking the blocking group.