	US 12,077,779 B2
	Feeder cell and method for growing gamma delta T cells by using same
Tai Gyu Kim, Seoul (KR); Hyun Jung Sohn, Seoul (KR); Hyun Woo Cho, Gyeonggi-do (KR); and Su Yeon Kim, Seoul (KR)
Assigned to The Catholic University of Korea Industry-Academic Cooperation Foundation, Seoul (KR)
Appl. No. 16/349,225
Filed by THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Seoul (KR)
PCT Filed Nov. 10, 2017, PCT No. PCT/KR2017/012701 § 371(c)(1), (2) Date May 10, 2019, PCT Pub. No. WO2018/088829, PCT Pub. Date May 17, 2018.
Claims priority of application No. 10-2016-0150338 (KR), filed on Nov. 11, 2016.
Prior Publication US 2019/0284532 A1, Sep. 19, 2019
Int. Cl. A61K 48/00 (2006.01); A61K 35/17 (2015.01); A61K 39/39 (2006.01); A61K 51/10 (2006.01); C07K 14/705 (2006.01); C12N 5/073 (2010.01); C12N 5/0783 (2010.01); C12N 5/09 (2010.01); C12N 5/10 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01)

CPC C12N 5/0638 (2013.01) [A61K 35/17 (2013.01); A61K 39/39 (2013.01); A61K 51/1042 (2013.01); C07K 14/70532 (2013.01); C07K 14/70578 (2013.01); C07K 14/70596 (2013.01); C12N 5/0603 (2013.01); C12N 5/0636 (2013.01); C12N 5/0694 (2013.01); C12N 5/10 (2013.01); G01N 33/505 (2013.01); G01N 33/56972 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01); C12N 2501/52 (2013.01); C12N 2510/00 (2013.01)]

3 Claims

1. A method of in vitro proliferating, γδ T cells, comprising:

stimulating isolated human PBMCs comprising γδ T cells with zoledronic acid and IL-2 for a first period to generate enriched γδ T cells, and then stimulating enriched γδ T cells in the isolated human PBMCs by co-culture with human leukocyte antigen (HLA) deficient feeder cells expressing 4-1BBL, CD80, CD83 and CD40L in the presence of IL-2 and without an anti-CD3 antibody for a second period to obtain proliferating γδ T cells,

wherein the feeder cells are prepared by transducing nucleic acids encoding a costimulatory molecule group consisting of 4-1BBL, CD80, CD83 and CD40L, into isolated K562 cells that do not express HLA.